The Mane Six are Fluttershy, Rarity, Pinkie Pie, Applejack, Rainbow Dash and their leader, Twilight Sparkle. They are Ponies, magical being from another dimension who resemble Earth equines. Inside each of the flows the power of the six Elements of Harmony: Laughter, Honesty, Generosity, Kindness, Loyalty, and Magic. Together, the six Elements form the Rainbow of Harmony, a magical beam of energy that can purify or destroy any enemy. The Mane Six each embody the essence of their Element: Pinkie Pie is cheerful and determined to bring joy to everyone, Fluttershy is kind and compassionate, Applejack doesn't like telling lies, Rainbow Dash always puts her friends above all else, Rarity wants to share her skills with everyone, and Twilight Sparkle has vast amounts of magical power.

The Mane Six are Raven's idols and role models, and Raven tries to be a wise, kind and gentle heroine like the Mane Six.